Transcription profiling of P.aeruginosa PA14 and P.aeruginosa PA14 metR::phoA
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ABSTRACT: Five biological repeats of P.aeruginosa PA14 and five biological repeats of P.aeruginosa PA14 metR::phoA were grown in liquid BM2 swarming media containing 62mM potassium phosphate buffer [pH7], 2 mM MgSO4, 10uM FeSO4, 0.4% (wt/vol) glucose, and 0.1% (wt/vol) casamino acids. Microarray experiments were done using microarray slides and protocols from TIGR on samples taken from mid-log phase (OD600 0.4-0.5)cells.
Project description:Four biological repeats of P.aeruginosa PA14 and four biological repeats of P.aeruginosa PA14 metR::phoA were grown in BM2 swarming media plates containing 62mM potassium phosphate buffer [pH7], 2 mM MgSO4, 10uM FeSO4, 0.4% (wt/vol) glucose, 0.1% (wt/vol) casamino acids and 0.5% agar. Microarray experiments were done using microarray slides and protocols from TIGR on samples harvested from the dendritic swarm colony growth, 2 to 3 mm of the swarm zone edge.
Project description:To determine what genes play a role in virulence of global regulator cbrA. RNA was isolated from 3 biological repeats of PA14 cbrA deletion mutant and 3 biological repeats of Pseudomonas P14 parent strain grown on BM2 swarming plates. RNA was isolated from the edge of the dendritic clonies of PA14 and the entire colony on the non-swarming cbrA mutant.
Project description:Five biological repeats of P.aeruginosa PAO1 and five biological repeats of P.aeruginosa PAO1 phoQ::xylE were grown in mineral medium BM2 using glucose as the carbon source and with 2mM MgSO4. Microarray experiments were done using microarray slides and protocols from TIGR on cells from mid-log phase.
Project description:Microarray were done on 4 independent cultures. PA01 was grown for 6 hours to late log growth phase (OD600 0.9-1.0) in liquid BM2-swarming media under shaking conditions(swimming) or for 18 hours at 37C on BM2-swarming plate containing 0.5% (w/v) agar and 0.1% casamino acids. Cells were harvested either by centrifugation(swimming) or by a sterile inoculating loop (swarming) and were resuspended n BM2-swarming media supplement with RNAprotect reagent (Qiagen)
Project description:RNA was isolated from five biological repeats of P.aeruginosa and five biological repeats of P.aeruginosa parR mutant grown in BM2 media contianing glucose as the carbon source, 2 mM MgSO4 and 4 ug/ml indolicidin. Microarray experiments were done using microarray slides and protocols from http://pfgrc.jcvi.org/index.php/microarray/protocols.html
Project description:To see the effects of tobramycin on Pseudomonas aeruginosa under anaerboc lethal conditions. RNA was isolated from 5 biological repeats of P.aeruginosa grown in cationic adjusted mueller hinton broth containing 15mM KNO3 and 5 biological repeats of P.aeruginosa grown in cationic adjusted mueller hinton broth containing 15mM KNO3 after being treated with 20 ug/ml tobramycin for 30 minutes.
Project description:To see the effect of lethal concentrations of tobramycin on Pseudomonas aeruginosa grown under aerobic conditions. RNA was isolated from 4 biological repeats of P.aeruginosa grown to mid-log in cationic adjusted mueller hinton broth containing 15mM KNO3 and 4 biological repeats of P.aeruginosa grown to mid-log in cationic adjusted mueller hinton broth containing 15mM KNO3 and treated with 2 ug/ml tobramycin for 30 minutes. null
Project description:5 Biological repeats of bacterial strain PAO1(parent, control) were compared to 5 biological repeats of PAO1_psrA::Tn5 knockout mutant, both strains were grown in BM2-glucose media containing 2mM MgSO4. Cells were grown to mid-log phase, OD600 0.50 Cells were immediately harvested and RNA was extracted
Project description:Five biological repeats of P.aeruginosa PAO1 in the absence of human LL37 and five biological repeats of P.aeruginosa presence of LL37 were grown in mineral medium in continuous-culture flow cells. Microarray experiments were done using microarray slides and protocols from TIGR on cells harvested from the biofilms after 4 days of growth.
Project description:To see the effect of sub-inhibitory concentrations of tobramycin on Pseudomonas aeruginosa grown under aerobic conditions. RNA was isolated form 4 biological repeats of P.aeruginosa grown to mid-log in cationic adjusted mueller hinton broth and 4 biological repeats of P.aeruginosa with the addition of 0.25ug/ml tobramycin in cationic adjusted mueller hinton broth. null