Transcription profiling of soybean nodulation by Bradyrhizobium japonicum

ABSTRACT: Legumes interact with nodulating bacteria that convert atmospheric nitrogen into ammonia for plant use. This nitrogen fixation takes place within root nodules that form after infection of root hairs by compatible rhizobia. Using cDNA microarrays, we monitored gene expression in soybean (Glycine max) inoculated with the nodulating bacterium Bradyrhizobium japonicum 4, 8, and 16 days after inoculation (dai), time points that coincided with nodule development and the onset of nitrogen fixation. This experiment identified several thousand genes that were differentially expressed in response to B. japonicum inoculation. Expression of 27 genes was analyzed by qRT-PCR and their expression patterns mimicked the microarray results confirming integrity of analyses. The microarray results suggest tha

ORGANISM(S): Glycine max

SUBMITTER: min li 

PROVIDER: E-GEOD-10340 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress