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Comparative expression analysis between SdrP (TTHA1359) deletion mutant and wild-type of T. thermophilus HB8

ABSTRACT: We observed the expression profile of the total mRNA of TTHA1359 deletion mutant of Thermus thermophilus HB8 strain grown in a rich (TR) medium at 70Â°C compared with that of wild type. Keywords: cell type comparison Four wild-type and mutant T. thermophilus HB8 strains were each pre-cultured at 70Â°C for 16 h in 3 ml of TR medium containing 0.8% polypeptone, 0.4% yeast extract, 0.2% NaCl, 0.4 mM CaCl2, and 0.4 mM MgCl2, which was adjusted to pH 7.2 with NaOH. The cells (2 ml) were inoculated into 1 liter of the same medium and then cultivated at 70Â°C. Cells were collected after 680 min, and then crude RNA was extracted. In order to compare mRNA expression in the TTHA1359 deletion mutant with that of wild type, the expression of each mRNA was analyzed on a GeneChip as described under Sample Description Sheet of each sample in this website.

ORGANISM(S): Thermus thermophilus HB8

SUBMITTER: Akeo Shinkai

PROVIDER: E-GEOD-10369 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Global gene expression mediated by Thermus thermophilus SdrP, a CRP/FNR family transcriptional regulator.

Agari Yoshihiro Y Kashihara Aiko A Yokoyama Shigeyuki S Kuramitsu Seiki S Shinkai Akeo A

Molecular microbiology 20080811 1

Thermus thermophilus SdrP is one of four cyclic AMP receptor protein (CRP)/fumarate and nitrate reduction regulator (FNR) family proteins from the extremely thermophilic bacterium T. thermophilus HB8. Expression of sdrP mRNA increased in the stationary phase during cultivation at 70 degrees C. Although the sdrP gene was non-essential, an sdrP-deficient strain showed growth defects, particularly when grown in a synthetic medium, and increased sensitivity to disulphide stress. The expression of se ...[more]

PMID: 18699868

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SuperSeries for the study of expression analysis of the SdrP (TTHA1359) in T. thermophilus HB8

Project description:This SuperSeries is composed of the following subset Series: GSE10368: Time course of the mRNA expression in wild-type Thermus thermophilus HB8 strain grown on a rich medium. GSE10369: comparative expression analysis between TTHA1359 deletion mutant and wild-type of T. thermophilus HB8 GSE11671: comparative expression analysis between logarithmic and stationary phase of T. thermophilus HB8 on a synthetic medium. Keywords: SuperSeries Refer to individual Series

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Time course of the mRNA expression in wild-type Thermus thermophilus HB8 strain grown on a rich medium.

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Time course of the mRNA expression after bacteriophage ÏYS40 infection in wild-type Thermus thermophilus HB8 strain.

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2010-10-15 | E-GEOD-21473 | biostudies-arrayexpress

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Project description:We observed the expression profile of the total mRNA of TTHA0101 deletion mutant of Thermus thermophilus HB8 strain grown in a rich (TT) medium at 70Â°C compared with that of wild type. Four wild-type and three mutant T. thermophilus HB8 strains were each pre-cultured at 70Â°C for 16 h in 3 ml of TT medium containing 0.8% polypeptone, 0.4% yeast extract, 0.2% NaCl, 0.4 mM CaCl2, and 0.4 mM MgCl2, which was adjusted to pH 7.2 with NaOH. The cells (2 ml) were inoculated into 1 liter of the same medium and then cultivated at 70Â°C. Cells were collected after 680 min, and then crude RNA was extracted. In order to compare mRNA expression in the TTHA0101 deletion mutant with that of wild type, the expression of each mRNA was analyzed on a GeneChip.

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