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Expression profile of TTHA0175-deficient Thermus thermophilus HB8 strain

ABSTRACT: In order to find function of TTHA0175 protein from T. thermophilus HB8, we observed the expression profile of the total mRNA of the TTHA0175-deficient strain grown in a rich medium at 70Â°C for 300, 480, and 600 min. Two TTHA0175-deficient T. thermophilus HB8 strains were each pre-cultured at 70Â°C for 16 h in 3 ml of TT medium containing 0.8% polypeptone, 0.4% yeast extract, 0.2% NaCl, 0.4 mM CaCl2, and 0.4 mM MgCl2, which was adjusted to pH 7.2 with NaOH. The cells (2 ml) were inoculated into 1 liter of the same medium and then cultivated at 70Â°C. Cells were collected after 300, 480, and 600 min, respectively, and then, the expression of each mRNA was analyzed.

ORGANISM(S): Thermus thermophilus HB8

SUBMITTER: Yoshihiro Agari

PROVIDER: E-GEOD-21471 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Time course of the mRNA expression in wild-type Thermus thermophilus HB8 strain grown in a rich medium (labeling:ENZO)

Project description:In order to observe altered expression profile of wild-type T. thermophilus HB8 strain grown in a rich (TT) medium at 70Â°C. we analyzed time-dependent mRNA expression profile of the total mRNA from the strain, which corresponds the logarithmic phase to the early stationary phase. Three wild-type T. thermophilus HB8 strains were pre-cultured at 70Â°C for 16 h in 3 ml of TT medium containing 0.8% polypeptone, 0.4% yeast extract, 0.2% NaCl, 0.4 mM CaCl2, and 0.4 mM MgCl2, which was adjusted to pH 7.2 with NaOH. The cells (2 ml) were inoculated into 1 liter of the same medium and then cultivated at 70Â°C. Cells were collected after 180, 240, 300, 360, 420, 480, 540, 600, 680, and 760 min, and then crude RNA was extracted. The expression of each mRNA at each time point was analyzed on a GeneChip.

2010-10-15 | E-GEOD-21473 | biostudies-arrayexpress

Expression profile of TTHA1438-deficient Thermus thermophilus HB8 strain

Project description:In order to find function of TTHA1438 protein from T. thermophilus HB8, we observed the expression profile of the total mRNA of the TTHA1438-deficient strain grown in a rich medium at 70Â°C for 240, 360, 480, 600 and 760 min. Two TTHA1438-deficient T. thermophilus HB8 strains were each pre-cultured at 70Â°C for 16 h in 3 ml of TT medium containing 0.8% polypeptone, 0.4% yeast extract, 0.2% NaCl, 0.4 mM CaCl2, and 0.4 mM MgCl2, which was adjusted to pH 7.2 with NaOH. The cells (2 ml) were inoculated into 1 liter of the same medium and then cultivated at 70Â°C. Cells were collected after 240, 360, 480, 600, 680 and 760 min, respectively, and then, the expression of each mRNA was analyzed.

2010-10-15 | E-GEOD-21198 | biostudies-arrayexpress

Comparative expression analysis between SdrP (TTHA1359) deletion mutant and wild-type of T. thermophilus HB8

Project description:We observed the expression profile of the total mRNA of TTHA1359 deletion mutant of Thermus thermophilus HB8 strain grown in a rich (TR) medium at 70Â°C compared with that of wild type. Keywords: cell type comparison Four wild-type and mutant T. thermophilus HB8 strains were each pre-cultured at 70Â°C for 16 h in 3 ml of TR medium containing 0.8% polypeptone, 0.4% yeast extract, 0.2% NaCl, 0.4 mM CaCl2, and 0.4 mM MgCl2, which was adjusted to pH 7.2 with NaOH. The cells (2 ml) were inoculated into 1 liter of the same medium and then cultivated at 70Â°C. Cells were collected after 680 min, and then crude RNA was extracted. In order to compare mRNA expression in the TTHA1359 deletion mutant with that of wild type, the expression of each mRNA was analyzed on a GeneChip as described under Sample Description Sheet of each sample in this website.

2010-05-25 | E-GEOD-10369 | biostudies-arrayexpress

Expression profile of TTHA1359-deficient Thermus thermophilus HB8 strain

Project description:In order to find function of TTHA1359 protein from T. thermophilus HB8, we observed the expression profile of the total mRNA of the sdrP-deficient strain grown in a rich medium at 70Â°C for 300, 480, and 600 min. TTHA1359-deficient T. thermophilus HB8 strains were each pre-cultured at 70Â°C for 16 h in 3 ml of TT medium containing 0.8% polypeptone, 0.4% yeast extract, 0.2% NaCl, 0.4 mM CaCl2, and 0.4 mM MgCl2, which was adjusted to pH 7.2 with NaOH. The cells (2 ml) were inoculated into 1 liter of the same medium and then cultivated at 70Â°C. Cells were collected after 680, 1120, and 1240 min, respectively, and then, the expression of each mRNA was analyzed.

2010-10-15 | E-GEOD-21472 | biostudies-arrayexpress

Time course of the mRNA expression in wild-type Thermus thermophilus HB8 strain grown on a rich medium.

Project description:We observed the expression profile of the total mRNA of wild-type Thermus thermophilus HB8 strain grown in a rich (TR) medium at 70Â°C. Keywords: time course Three wild-type T. thermophilus HB8 strains were pre-cultured at 70Â°C for 16 h in 3 ml of TR medium containing 0.8% polypeptone, 0.4% yeast extract, 0.2% NaCl, 0.4 mM CaCl2, and 0.4 mM MgCl2, which was adjusted to pH 7.2 with NaOH. The cells (2 ml) were inoculated into 1 liter of the same medium and then cultivated at 70Â°C. Cells were collected after 180, 240, 300, 360, 420, 480, 540, 600, 680, and 760 min, and then crude RNA was extracted. The expression of each mRNA at each time point was analyzed on a GeneChip as described under Sample Description Sheet of each sample in this website.

2010-05-25 | E-GEOD-10368 | biostudies-arrayexpress

Comparative expression analysis between TTHB212 deletion mutant and wild-type of T. thermophilus HB8

Project description:We observed the expression profile of the total mRNA of TTHB212 deletion mutant of Thermus thermophilus HB8 strain grown in a rich (TR) medium at 70Â°C compared with that of wild type. Keywords: cell type comparison Three wild-type and mutant T. thermophilus HB8 strains were each pre-cultured at 70Â°C for 16 h in 3 ml of TR medium containing 0.8% polypeptone, 0.4% yeast extract, 0.2% NaCl, 0.4 mM CaCl2, and 0.4 mM MgCl2, which was adjusted to pH 7.2 with NaOH. The cells (2 ml) were inoculated into 1 liter of the same medium and then cultivated at 70Â°C. Cells were collected after 5 h for wild-type and 7 h for TTHB212 deletion mutant, respectively, and then crude RNA was extracted. In order to compare mRNA expression in the TTHB212 deletion mutant with that of wild type, the expression of each mRNA was analyzed on a GeneChip as described under Sample Description Sheet of each sample in this website.

2010-05-25 | E-GEOD-10371 | biostudies-arrayexpress

SuperSeries for the study of expression analysis of the SdrP (TTHA1359) in T. thermophilus HB8

Project description:This SuperSeries is composed of the following subset Series: GSE10368: Time course of the mRNA expression in wild-type Thermus thermophilus HB8 strain grown on a rich medium. GSE10369: comparative expression analysis between TTHA1359 deletion mutant and wild-type of T. thermophilus HB8 GSE11671: comparative expression analysis between logarithmic and stationary phase of T. thermophilus HB8 on a synthetic medium. Keywords: SuperSeries Refer to individual Series

2010-05-25 | E-GEOD-10370 | biostudies-arrayexpress

Expression profile of TTHB186-deficient Thermus thermophilus HB8 strain

Project description:In order to find function of TTHB186 protein from T. thermophilus HB8, we observed the expression profile of the total mRNA of the TTHB186-deficient strain grown in a rich medium at 70Â°C for 360, and 480 min. Three TTHB186-deficient T. thermophilus HB8 strains were each pre-cultured at 70Â°C for 16 h in 3 mL of TT medium containing 0.8% polypeptone, 0.4% yeast extract, 0.2% NaCl, 0.4 mM CaCl2, and 0.4 mM MgCl2, which was adjusted to pH 7.2 with NaOH. The cells (2 mL) were inoculated into 500 mL of the same medium and then cultivated at 70Â°C. Cells were collected after 360, and 480 min, respectively, and then, the expression of each mRNA was analyzed.

2010-10-15 | E-GEOD-21457 | biostudies-arrayexpress

Expression profile of TTHA1634-deficient Thermus thermophilus HB8 strain

Project description:In order to find function of TTHA1634 protein from T. thermophilus HB8, we observed the expression profile of the total mRNA of the TTHA1634-deficient strain grown in a rich medium at 70Â°C for 420, and 600 min. Two TTHA1634-deficient T. thermophilus HB8 strains were each pre-cultured at 70Â°C for 16 h in 3 mL of TT medium containing 0.8% polypeptone, 0.4% yeast extract, 0.2% NaCl, 0.4 mM CaCl2, and 0.4 mM MgCl2, which was adjusted to pH 7.2 with NaOH. The cells (2 mL) were inoculated into 1 L of the same medium and then cultivated at 70Â°C. Cells were collected after 420, and 600 min, respectively, and then, the expression of each mRNA was analyzed.

2010-10-15 | E-GEOD-21460 | biostudies-arrayexpress

Comparative expression analysis between TTHA0101-deficient mutant and wild-type of Thermus thermophilus HB8

Project description:We observed the expression profile of the total mRNA of TTHA0101 deletion mutant of Thermus thermophilus HB8 strain grown in a rich (TT) medium at 70Â°C compared with that of wild type. Four wild-type and three mutant T. thermophilus HB8 strains were each pre-cultured at 70Â°C for 16 h in 3 ml of TT medium containing 0.8% polypeptone, 0.4% yeast extract, 0.2% NaCl, 0.4 mM CaCl2, and 0.4 mM MgCl2, which was adjusted to pH 7.2 with NaOH. The cells (2 ml) were inoculated into 1 liter of the same medium and then cultivated at 70Â°C. Cells were collected after 680 min, and then crude RNA was extracted. In order to compare mRNA expression in the TTHA0101 deletion mutant with that of wild type, the expression of each mRNA was analyzed on a GeneChip.

2010-10-15 | E-GEOD-24184 | biostudies-arrayexpress

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