In vitro dormancy achieved by multiple stresses in Mycobacterium tuberculosis

ABSTRACT: The human pathogen, Mycobacterium tuberculosis, develops a dormant infection, in which organisms survive within the body. We established a unique in vitro dormancy model based on the characterization of drug-resistance to INH and rifampin. M. tuberculosis cells were maintained in controlled and defined multiple stress conditions with low oxygen (5% dissolved oxygen tension), acid (pH 5.) along with glycerol-deprived medium conditions. To monitor gene expression changes in M. tuberculosis in response to the multiple stresses, we performed microarray analysis at the time point of 1, 2, 3, 6, and 12days after treatment. M. tuberculosis adapting to multiple stresses displayed characteristics associated with persistence in vivo, including entry into a non-replicative state and the repression of

ORGANISM(S): Mycobacterium tuberculosis H37Rv

SUBMITTER: ChangMuk Lee 

PROVIDER: E-GEOD-10391 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress