Simultaneous gene expression profiling in human macrophages infected with Leishmania major parasites using SAGE

ABSTRACT: Leishmania (L) are intracellular protozoan parasites which are able to survive and replicate within the harsh and potentially hostile phago-lysosomal environment of mammalian mononuclear phagocytes. A complex interplay then takes place between the macrophage (MM-NM-&) striving to eliminate the pathogen and the parasite struggling for its own survival. To investigate, at the transcriptional level, this host-parasite conflict in the context of monocyte-derived human MM-NM-&s (MDM) infection by L. major metacyclic promastigotes, the quantitative technique of serial analysis of gene expression (SAGE) was used. After extracting mRNA from resting human MM-NM-&s, Leishmania-infected human MM-NM-&s and L. major parasites, three SAGE libraries were constructed and sequenced generating up to 28,17

ORGANISM(S): Leishmania major

SUBMITTER: Dhafer Laouini 

PROVIDER: E-GEOD-10442 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress