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Transcription profiling of human neuropheres cultured from fetal brain tissue

ABSTRACT: Three week old neurospheres cultured from 18, 20 and 22 week old human fetal brain tissue, generated from epidermal growth factor, platelet-derived growth factor, and platelet-derived growth factor and neurotrophin-3. Experiment Overall Design: this experiment include 9 samples and 18 replicates

ORGANISM(S): Homo sapiens

SUBMITTER: OGIC Info Ontario Genomics Innovation Centre (OGIC)

PROVIDER: E-GEOD-10691 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Human Neuropheres

Project description:Three week old neurospheres cultured from 18, 20 and 22 week old human fetal brain tissue, generated from epidermal growth factor, platelet-derived growth factor, and platelet-derived growth factor and neurotrophin-3. Keywords: neurosphere development

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Transcription profiling of mouse neurospheres cultured from the embryonic ganglia

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Transcription profiling of mouse liver from tumor bearing animals by TSU68 treatment

Project description:The aim of this study was to investigate the inhibitory effect of TSU68, a tyrosine kinase inhibitor of vascular endothelial growth factor receptor 2 (VEGFR2), platelet-derived growth factor receptor beta (PDGFRÎ²) and fibroblast growth factor receptor 1 (FGFR1), on colon cancer liver metastasis and to test the hypothesis that TSU68 modulates the microenvironment in the liver before the formation of metastasis. Experiment Overall Design: The human colon cancer TK-4 was implanted orthotopically into cecal walls of 6-week-old male BALB/c nu/nu mice (Clea Japan, Tokyo, Japan). The animals were treated with TSU68 (400 mg/kg/day, twice-daily, p.o.) or vehicle from 7 days after orthotopic implantation. After one week of drug administration, livers were removed and total RNA was extracted. Experiment Overall Design: We established four different groups of mice; non-tumor-bearing and treated with vehicle alone (NT-Co), non-tumor-bearing and treated with TSU68 (NT-TSU), tumor-bearing and treated with vehicle (T-Co), and tumor-bearing and treated with TSU68 (T-TSU). NT-Co, T-Co and T-TSU group were applied to microarray analysis.

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