SiRNA of human MCF10A eIF4GI depleted cells vs. controls links nutrient sensing by mTOR to cell proliferation and inhibition of autophagy

ABSTRACT: Translation initiation factors have complex functions in cells which are not yet understood. We show that depletion of initiation factor eIF4GI only modestly reduces overall protein synthesis in cells, but phenocopies nutrient-starvation or inhibition of protein kinase mTOR, a key nutrient sensor. eIF4GI depletion impairs cell proliferation, bioenergetics and mitochondrial activity, thereby promoting autophagy. Translation of mRNAs involved in cell growth, proliferation and bioenergetics were selectively inhibited by reduction of eIF4GI, whereas mRNAs encoding proliferation inhibitors and catabolic pathway factors were increased. Depletion or over-expression of other eIF4G family members did not recapitulate these results. The majority of mRNAs that were translationally impaired with eIF4G

ORGANISM(S): Homo sapiens

SUBMITTER: Robert Schneider 

PROVIDER: E-GEOD-11011 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress