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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Transcript Analysis of Human Mammary Cell Subsets using SAGE

ABSTRACT: Highly purified subpopulations of primitive bipotent and committed luminal progenitor cells as well as mature luminal and myoepithelial cells from normal human mammary tissue were isolated and compared their transcriptomes which were obtained using PCR-Long-SAGE technology. Keywords: mammary progenitors, stem cells, Notch signaling, gene expression Four SAGE libraries were constructed on RNA samples extracted from highly purified subpopulations of primitive bipotent and committed luminal progenitor cells as well as mature luminal and myoepithelial cells isolate from a normal human mammary tissue.

ORGANISM(S): Homo sapiens

SUBMITTER: BCGSC BC Cancer Agency

PROVIDER: E-GEOD-11387 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Transcriptome analysis of the normal human mammary cell commitment and differentiation process.

Raouf Afshin A Zhao Yun Y To Karen K Stingl John J Delaney Allen A Barbara Mary M Iscove Norman N Jones Steven S McKinney Steven S Emerman Joanne J Aparicio Samuel S Marra Marco M Eaves Connie C

Cell stem cell 20080701 1

Mature mammary epithelial cells are generated from undifferentiated precursors through a hierarchical process, but the molecular mechanisms involved, particularly in the human mammary gland, are poorly understood. To address this issue, we isolated highly purified subpopulations of primitive bipotent and committed luminal progenitor cells as well as mature luminal and myoepithelial cells from normal human mammary tissue and compared their transcriptomes obtained using three different methods. El ...[more]

PMID: 18593563

Similar Datasets

Transcription profiling of human primitive bipotent and committed luminal progenitor cells as well as mature luminal and myoepithelial cells from normal mammary tissue

Project description:Highly purified subpopulations of primitive bipotent and committed luminal progenitor cells as well as mature luminal and myoepithelial cells from normal human mammary tissue were isolated and compared their transcriptomes obtained using the Affymetrix GeneChip Human X3P Array. Experiment Overall Design: 12 Afffymetrix array hybridizations were performed on RNA samples extracted from highly purified subpopulations of primitive bipotent and committed luminal progenitor cells as well as mature luminal and myoepithelial cells isolate from 3 different normal human mammary tissue.

2008-06-20 | E-GEOD-11394 | biostudies-arrayexpress

Transcript Analysis of Human Mammary Cell Subsets using SAGE

2008-05-10 | GSE11387 | GEO

Transcript Analysis of Human Mammary Cell Subsets by Chip Technology

2008-05-10 | GSE11394 | GEO

Global gene expresion profile of phenotypically distinct human mammary epithelial subpopulations of various developmental age

Project description:To get molecular insight into age- and compartment-specific changes in telomere maintenance and associated properties in human mammary gland, we analyzed distinct subsets of normal human mammary epithelial cells. The cells were isolated by fluorescent activated cell sorting (FACS) directly from mammary tissue obtained from normal women undergoing reduction mammoplasties with concomitant removal of hematopoietic and endothelial cells by depletion of CD45pos and CD31pos cells. The three epithelial cell populations then isolated were: (i) CD49fhiEPCAMneg/low cells, (ii) CD49fposEPCAMpos cells and (iii) CD49fnegEPCAMpos cells. The CD49fhiEPCAM-/low cells are selectively enriched in mammary stem cells with functional mammary gland regenerating activity in suitably transplanted immunodeficient mice, bipotent progenitors that form colonies of adherent myoepithelial and luminal cells in vitro, myoepithelial-restricted progenitors that form colonies of exclusively adherent myoepithelial cells in vitro, and mature myoepithelial cells that are not clonogenic (collectively referred to as basal cells, BCs). The CD49fposEPCAMpos cells are selectively enriched in luminal progenitors (referred to as luminal progenitors, LPs); and the CD49fnegEPCAMpos cells are selectively enriched in mature luminal cells (referred to as luminal cells, LCs). Differences in gene expression in general and telomere associated genes in particular were elucidated by analyzing mammary epithelial subpopulations. Total RNA was isolated from 24 samples obtained from FACS purification of mammary epithelial subpopulations from 9 reduction mammoplasty breast tissues. Global gene expression profiling was performed by array.

2013-07-26 | E-GEOD-37223 | biostudies-arrayexpress

Expression data of pre-stasis cultured HMEC

Project description:We have generated a large collection of normal human mammary epithelial cell strains from women aged 16 to 91 years, derived from primary tissues, to enable functional and molecular interrogation of aging. We demonstrate in finite-lifespan cultured and uncultured epithelial cells that aging is associated with reduction of myoepithelial cells and with increases in luminal cells expressing keratin 14 and integrin Î±6, traits that are expressed exclusively in myoepithelial cells in women under 30. We find that changes to the luminal lineage result from age-dependent expansion of multipotent progenitors that bear defects resulting in incompletely differentiated luminal cells. These findings were verified in vivo in normal breast tissues. Myoepithelial cells have been suggested to act as tumor suppressors, and progenitor cells are implicated as the etiological roots of mammary carcinomas. Thus with aging there is a shift in the balance of luminal/myoepithelial lineages, and changes in the functional spectrum of multipotent progenitors, which presages increased potential for malignant transformation. Finite lifespan pre-stasis HMEC from specimens from reduction mammoplasties of 7 patients of different age were collected. The expression data was queried for different biological replicates and at different passages. For strain 240, luminal and myoepithelial cells were collected using flow cytometry.

2012-05-02 | E-GEOD-37485 | biostudies-arrayexpress

Progesterone and estrogen receptors segregate into different cell subpopulations in the normal human breast

Project description:Gene expression profiles of normal human mammary epithelial cell subsets enriched for luminal or myoepithelial characteristics on the basis of EpCAM and CD49f expression. Transcription profiles were compared between luminal- and myoepithelial-enriched human MEC subsets following 3D culture from 4 individuals. Gene expression profiles of normal human mammary epithelial cell subsets enriched for mature, luminal progenitor or bipotent progenitor-enriched characteristics on the bais of CD49f, MUC1, CD10, CD133 and Thy1 expression Transcription profiles were compared between unsorted, mature, luminal progenitor-enriched and bipotent progenitor-enriched human MEC subsets following 3D culture from 2 individuals. Four replicates of luminal-enriched human MECs and 3 replicates of myoepithelial-enriched MECs Transcription profiles of two replicates of unsorted, mature, luminal progenitor-enriched and bipotent progenitor-enriched human MEC subsets were analysed following 3D culture

2012-08-17 | E-GEOD-31429 | biostudies-arrayexpress

Transcript Analysis of Human Mammary Cell Subsets

Project description:This SuperSeries is composed of the following subset Series: GSE11387: Transcript Analysis of Human Mammary Cell Subsets using SAGE GSE11394: Transcript Analysis of Human Mammary Cell Subsets by Chip Technology Keywords: SuperSeries Refer to individual Series

2008-05-09 | E-GEOD-11395 | biostudies-arrayexpress

Global gene expresion profile of phenotypically distinct human mammary epithelial subpopulations of various developmental age

2013-07-26 | GSE37223 | GEO

Progesterone and estrogen receptors segregate into different cell subpopulations in the normal human breast

2012-08-17 | GSE31429 | GEO

Gene expression profile of normal human mammary epithelial stem/progenitor and myoepithelial cells

Project description:There is increasing evidence that breast and other cancers originate from and are maintained by a small fraction of stem/progenitor cells with self-renewal properties. Whether such cancer stem/progenitor cells originate from normal stem cells based on initiation of a de novo stem cell program, by reprogramming of a more differentiated cell type by oncogenic insults or both remains unresolved. A major hurdle in addressing these issues is lack of immortal human stem/progenitor cells that can be deliberately manipulated in vitro. We present evidence that normal and human telomerase reverse transcriptase (hTERT)-immortalized human mammary epithelial cells (hMECs) isolated and maintained in DFCI-1 medium retain a fraction with progenitor cell properties. These cells co-express basal, luminal and stem/progenitor cell markers. Clonal derivatives of progenitors co-expressing these markers fall into two distinct types: K5+/K19- (Type I) and K5+/K19+ (Type II). We show that both types of progenitor cells have self-renewal and differentiation ability. Through microarray analysis, we want to identify genes and pathways linked to human mammary epithelial stem/progenitor cell self-renewal and differentiation. Normal human mammary epithelial cells (hMECs) were isolated from Reduction Mammoplasty and immortalized by hTERT. Type I K5+/K19- and Type II K5+/K19+ cell colonies were isolated from hTERT-immortalized hMECs and cultured in MEGM medium for self-renewal and differentiation. Total RNA isolated from Type I, Type II, and differentiated Myoepithelial (Myo) cells were used on Affymetrix microarrays.

2010-07-29 | E-GEOD-22580 | biostudies-arrayexpress

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