Transcription profiling time series of the response to estradiol by human osteosarcoma cells expressing estrogen receptors alpha or beta

ABSTRACT: Estrogens exert many important effects in bone, a tissue that contains both estrogen receptors alpha and beta (ERalpha and ERbeta). To compare the actions of these receptors, we generated U2OS human osteosarcoma cells stably expressing ERalpha or ERbeta, at levels comparable to those in osteoblasts, and we characterized their response to estradiol (E2) over time using Affymetrix GeneChip microarrays to determine the expression of approximately 12,000 genes, followed by quantitative PCR verification of the regulation of selected genes. Of the ca. 100 regulated genes we identified, some were stimulated by E2 equally through ERalpha and ERbeta, whereas others were selectively stimulated via ERalpha or ERbeta. The E2-regulated genes showed three distinct temporal patterns of expression over th

ORGANISM(S): Homo sapiens

SUBMITTER: Fabio Stossi 

PROVIDER: E-GEOD-1153 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress