Transcription profiling of mouse after ventilator-induced lung injury: role of Akt signaling

ABSTRACT: Rationale: Using an ortholog candidate gene approach, we previously identified GADD45a (growth arrest and DNA damage-inducible gene 45a) as a potential novel candidate involved in ventilator-induced lung injury (VILI). Objectives: We investigated whether genetically engineered mice with GADD45a deletion were more susceptible to ventilator- or endotoxin (LPS)-induced lung injury. We employed genomic strategies to explore the mechanistic involvement of GADD45a in inflammatory lung injury. Methods: Wild-type C57Bl/6 and GADD45a-/- mice were phenotyped for lung injury following exposure to high tidal volume ventilation (VILI) or intratracheal LPS. Whole lung homogenates were utilized for gene expression studies of ventilated animals and spontaneously breathing controls. To explore the conseque

ORGANISM(S): Mus musculus

SUBMITTER: Yong Huang 

PROVIDER: E-GEOD-11662 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress