Experimental analysis of oligonucleotide microarray design criteria to detect deletions by CGH

ABSTRACT: BACKGROUND: Microarray comparative genomic hybridization (CGH) is currently one of the most powerful techniques to measure DNA copy number in large genomes. In humans, microarray CGH is widely used to assess copy number variants in healthy individuals and copy number aberrations associated with various diseases, syndromes and disease susceptibility. In model organisms such as Caenorhabditis elegans (C. elegans) the technique has been applied to detect mutations, primarily deletions, in strains of interest. Although various constraints on oligonucleotide properties have been suggested to minimize non-specific hybridization and improve the data quality, there have been few experimental validations for CGH experiments. For genomic regions where strict design filters would limit the coverage

ORGANISM(S): Homo sapiens

SUBMITTER: Stephane Flibotte 

PROVIDER: E-GEOD-12208 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress