Gene expression profiles of human promyelocytic leukemia cell lines exposed to six volatile organic compounds

ABSTRACT: The gene expression profiles of HL-60 cells were determined for two doses (IC20 and IC50) of three chemicals (ethylbenzene, tricholoretylene and dichloromethane) and for the IC50 doses of 3 other chemicals (benzene, toluene and o-xylene) at a 3 h time point. Assays were performed in triplicate for each chemical/dose, and individual gene-expression profiles were determined for each chemical at the tested concentration doses. Gene expression analysis was conducted on the RNA samples using 35-K whole human genome microarray (Operon Biotechnologies, Inc. Germany). Three independent experiments were performed for each chemical (N = 6), simultaneously. Labeling and hybridization were performed using the instructions of the Platinum Biochip Reagent Kit (GenoCheck Co. Ltd., Korea). This was follow

ORGANISM(S): Homo sapiens

SUBMITTER: Sailendra Sarma 

PROVIDER: E-GEOD-12353 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress