Transcription profiling of human colorectal cancer cell lines treated with O-glycan inhibitors generate aryl-glycans, induce apoptosis, and inhibit growth

ABSTRACT: Our studies provide direct evidence that O-glycosylation pathways play a role in the regulation of cell growth through apoptosis and proliferation pathways. Eight small molecular weight analogues of the GalNAc-alpha-1-O-serine/threonine structure based on 1-benzyl-2-acetamido-2- deoxy-alpha-O-D-galactopyranoside have been synthesised and tested in 5 human colorectal cancer cell lines. Three inhibitors, 1-benzyl-2-acetamido-2-deoxy-alpha-O-D-galactopyranoside and the corresponding 2-azido- and C-glycoside analogues, were screened in two colorectal cancer cell lines at 0.5mM and showed induction of apoptosis. Proliferation was down regulated in the same two cell lines with all three inhibitors, as detected by Ki67 staining and gene array. Treatment both cell lines with inhibitors led to chan

ORGANISM(S): Homo sapiens

SUBMITTER: Andreas Klein 

PROVIDER: E-GEOD-12422 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress