Transcription profiling of Arabidopsis of til1-1 mutant plants heat shock response

ABSTRACT: Several lipocalin genes from higher plants were shown to be responsive to both high and low temperature stresses and have been named as temperature-induced lipocalin (Til). In this study, a reverse genetic approach was taken to elucidate the role of Arabidopsis Til1 (At5g58070) in thermotolerance. We showed that Til1 proteins was constitutively expressed and increased significantly after heat shock treatment. A T-DNA knockout line of Til1, designated as til1-1, could not produce Til1 and showed severe defects in basal and acquired thermotolerance. Introducing a wild type copy of Til1 gene into til1-1 complemented the mutant phenotype. Over-expression of Til1 in the wild type plant did not enhance thermotolerance. Til1 is peripherally associated with plasma membrane, suggesting a regu

ORGANISM(S): Arabidopsis thaliana

SUBMITTER: Yee-yung Charng 

PROVIDER: E-GEOD-12619 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress