Transcriptional profile asm gene cluster of high Ansamitocin P3 producing mutants derived from A. pretiosum

ABSTRACT: This series of microarry is the study of the transcriptional profile asm gene clusster of high Ansamitocin P3 producing mutants derived from isolating rifampicin resistant mutants. Background The Actinomycete Actinosynnema pretiosum ssp. auranticum has commercial importance due to its production of ansamitocin P-3 (AP-3), a potent antitumor agent. One method to improve the productivity of A. pretiosum is to genetically alter the regulation of ansamitocin biosynthesis by manipulating selected genes. To identify potential targets for genetic engineering, the transcriptional profile of an A. pretiosum mutant with enhanced AP-3 yield was compared to the parental strain. High-producing mutants were isolated from rifampicin-resistance screens using a plate-based bioassay. Transcriptional pr

ORGANISM(S): Actinosynnema pretiosum

SUBMITTER: Peter Nissom 

PROVIDER: E-GEOD-12739 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress