Mutation of the RNA polymerase M-NM-2-subunit gene promotes hetero-to-homo conversion of M-CM-^_-lactam resistance of methicillin-resistant Staphylococcus aureus

ABSTRACT: Three types of phenotypic expression of M-CM-^_-lactam resistance has been reported in MRSA: heterogeneous-, homogeneous-, and Eagle-type resistance. Heterogeneous to homogeneous (hetero-to-homo) conversion of M-CM-^_-lactam resistance is postulated to be caused by a chromosomal mutation (chr*) together with mecA-gene expression. The Eagle-type resistance is a special pattern of chr* expression in the pre-MRSA strain N315 under the strong mecI-gene mediated repression of mecA gene transcription. Here, for the identification of chr*, experiments were conducted using an in-vitro derived homogeneously imipenem-resistant MRSA strain N315M-bM-^HM-^FIPH5 (M-bM-^HM-^FIPH5). The strain was selected with imipenem 8 mg/L from the heterogeneously imipenem-resistant MRSA strain N315M-bM-^HM-^FIP (M-bM

ORGANISM(S): Staphylococcus aureus

SUBMITTER: tomomi hishinuma 

PROVIDER: E-GEOD-42218 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress