A Single Amino-Acid Substitution of RNA Polymerase M-CM-^_ subunit Confers A Novel Class of VISA phenotype on hVISA Strain Mu3

ABSTRACT: hVISA clinical strain Mu3 spontaneously generates VISA at an extremely high frequency (1x10-6 or greater) within its cell population. The VISA-converted mutant strains generally grow slower than their parent hVISA strain, but they usually form colonies on vancomycin-containing agar plates after 48 hours of incubation on population analysis. However, we noticed a curious group of VISA mutants whose colony formation is much more delayed and observed as discrete colonies only after 96 hours incubation. They have extremely prolonged lag-phase and doubling-times, but recorded vancomycin MICs of 8 to 24 mg/L when determined after 48 to 96 hours of incubation. We established strain Mu3-6RS having vancomycin MIC of 12 mg/L (at 72h) as a representative of the M-bM-^@M-^Xslow VISAM-bM-^@M-^Y (sVISA

ORGANISM(S): Staphylococcus aureus

SUBMITTER: tomomi hishinuma 

PROVIDER: E-GEOD-50826 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress