A mutation of RNA polymerase β’ subunit (RpoC) converts heterogeneously vancomycin-intermediate S. aureus (hVISA) into ‘slow-VISA’ (sVISA)

ABSTRACT: Various mutations in the rpoB gene, which encodes the RNA polymerase beta subunit, are associated with increased vancomycin (VAN) resistance in vancomycin-intermediate S. aureus (VISA) and hetero-VISA (hVISA) strains. We reported that rpoB mutations are also linked to the expression of the recently found slow VISA (sVISA) phenotype (Saito et al 2014 AAC). Because RpoC and RpoB are components of RNA polymerase, we examined the effect of rpoC(P440L) mutation on the expression of the sVISA phenotype in the Mu3fdh2*V6-5 strain (V6-5), which was derived from a previously reported hVISA strain with the VISA phenotype. V6-5 had an extremely prolonged doubling time (72.2 min) and high vncomycin MIC (16 mg/L). However, the phenotype of V6-5 was unstable, and the strain frequently reverted to hVISA

ORGANISM(S): Staphylococcus aureus

SUBMITTER: tomomi hishinuma 

PROVIDER: E-GEOD-65338 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress