Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

0

Transcriptome profiles of a riboflavin-producing B.subtilis RH33 and wild type B.subtilis 168

ABSTRACT: The transcriptome profiles of a riboflavin-producing recombinant Bacillus subtilis RH33 and wild type Bacillus subtilis 168 were compared using DNA microarrays to identify the target genes for further enhancing riboflavin production. Transcriptome profiles of a riboflavin-producing B. subtilis RH33 were compared with those of the wild-type B. subtilis 168 as a control strain during exponentially growing period on LBG medium. These conditions corresponded to the state of high riboflavin production, which was growth related. For each comparison, two independent cultivations on LB medium with 1% glucose were performed, and exponentially growing cells (OD600 3–6) were harvested. The synthesis of cDNA and biotin-labeled cRNA were carried out exactly as described in the Affymetrix GeneChip Expression Analysis Technical Manual (2000).

ORGANISM(S): Bacillus subtilis subsp. subtilis str. 168

SUBMITTER: Shuobo Shi 

PROVIDER: E-GEOD-12873 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

Json Xml
altmetric image

Publications

Transcriptome analysis guided metabolic engineering of Bacillus subtilis for riboflavin production.

Shi Shuobo S   Chen Tao T   Zhang Zhigang Z   Chen Xun X   Zhao Xueming X  

Metabolic engineering 20090513 4-5

A comparative transcriptome profiling between a riboflavin-producing Bacillus subtilis strain RH33 and the wild-type strain B. subtilis 168 was performed, complemented with metabolite pool and nucleotide sequence analysis, to rationally identify new targets for improving riboflavin production. The pur operon (purEKBCSQLFMNHD) together with other PurR-regulated genes (glyA, guaC, pbuG, xpt-pbuX, yqhZ-folD, and pbuO) was all down-regulated in RH33, which consequently limited the supply of the ribo  ...[more]

Similar Datasets

Unknown Transcriptome profiles of a riboflavin-producing B.subtilis RH33 and wild type B.subtilis 168
2009-09-20 | GSE12873 | GEO
Unknown Bacillus subtilis 168 moenomycin stimulon
2011-06-17 | GSE30001 | GEO
Unknown Expression data from Bacillus subtilis 168 during growth
2013-04-10 | GSE45933 | GEO
Transcriptomics Expression data from Bacillus subtilis 168 during growth
2013-04-10 | E-GEOD-45933 | biostudies-arrayexpress
Transcriptomics Bacillus subtilis 168 moenomycin stimulon
2011-06-17 | E-GEOD-30001 | biostudies-arrayexpress
Transcriptomics Bacillus subtilis WalR (YycF) ChIP-chip Bacillus subtilis; Bacillus subtilis subsp. subtilis str. 168
2015-01-26 | E-GEOD-65272 | biostudies-arrayexpress
Transcriptomics Expression analysis of sporulating Bacillus subtilis strain 168 deleted for prpE gene
2013-07-08 | E-GEOD-44125 | biostudies-arrayexpress
Unknown RNA-seq established gene expression data to determine the effects of various stresses on Bacillus subtilis str.168
2023-03-03 | GSE226559 | GEO
Transcriptomics Bacillus subtilis 168 cells: ccpN mutant and ccpN-yqfL double mutant analysis
2008-07-01 | E-GEOD-11937 | biostudies-arrayexpress
Unknown Transcriptomic analysis of VBNC cells of osmostressed Bacillus subtilis 168
2022-07-02 | GSE207180 | GEO