Myc influences embryonic stem cell pluripotency by regulating miRNAs

ABSTRACT: Previous studies have demonstrated that the c-Myc transcription factor regulates and cooperates with oncogenic miRNAs to accelerate tumorigenesis. Much recent work has shown that Myc plays an important role in stem cell pluripotency. These findings have prompted us to determine whether Myc regulates embryonic stem cell pluripotency through miRNAs. We have employed both miRNA- and expression- profiling to demonstrate that Myc up- or down-regulates a subset of miRNAs, which in turn influence expression of groups of genes involved in lineage differentiation. Chromatin immunoprecipitation analysis indicates that c-Myc binds proximal to the coding regions of these miRNAs in a dose dependent manner. Moreover, introduction of Myc-targeted miRNAs into murine ES cells significantly retards differen

ORGANISM(S): Mus musculus

SUBMITTER: Aimee Jackson 

PROVIDER: E-GEOD-13127 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress