Application of a translational profiling approach for the comparative analysis of CNS cell types.

ABSTRACT: Comparative analysis can provide important insights into complex biological systems. As demonstrated in the accompanying paper, Translating Ribosome Affinity Purification (TRAP), permits comprehensive studies of translated mRNAs in genetically defined cell populations following physiological perturbations. To establish the generality of this approach, we present translational profiles for twenty four CNS cell populations, and identify known cell-specific and enriched transcripts for each population. We report thousands of cell-specific mRNAs that were not detected in whole tissue microarray studies, and provide examples that demonstrate the benefits deriving from comparative analysis. To provide a foundation for further biological and in silico studies, we provide a resource of sixteen tra

ORGANISM(S): Mus musculus

SUBMITTER: Nathaniel Heintz 

PROVIDER: E-GEOD-13379 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress