Myxococcus xanthus cells: wild-type vs. ntrC mutant gene expression during development

ABSTRACT: Transcriptional profiling of Myxococcus xanthus comparing wild-type and mutant gene expression at different developmental time points The mutants in our study, nla4, nla18, nla6 and nla28 are strains that carry a mutation in the corresponding genes nla4 (MXAN2516), nla18 (MXAN3692), nla6(MXAN4042), and nla28(MXAN1167). (MXAN numbers are the conventional gene names; nlas are common names). All four of these genes are response regulators of the ntrC family of transcriptional activators. The mutations were made by insertion of a plasmid (pCR2.1TOPO, invitrogen)into the particular activator gene. Plasmids used carry a region of homology (usually 350-500 bp in length) to the sequence of the activator gene coresponding to the highly conserved central domain that is characteristic of these ntr

ORGANISM(S): Myxococcus xanthus

SUBMITTER: Anthony Garza 

PROVIDER: E-GEOD-13523 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress