Gene expression during early sporulation in Phytophthora infestans

ABSTRACT: Transcriptional changes during asexual sporangia formation by the late blight pathogen Phytophthora infestans were identified using microarrays representing 15,650 genes and RNA from sporulation time-courses, purified spores, and sporulation-defective strains. Results were confirmed by reverse transcription-polymerase chain reaction analyses of sporulation on artificial media and infected tomato. During sporulation, about 12% of genes were found to be up-regulated and 5% down-regulated. The most prevalent induced genes had functions in signal transduction, flagella assembly, cellular organization, metabolism, and molecular or vesicular transport. Distinct patterns of expression were discerned based on the kinetics of mRNA induction and their persistence in sporangia. For example, most

ORGANISM(S): Phytophthora infestans

SUBMITTER: Howard Judelson 

PROVIDER: E-GEOD-13580 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress