Prognostic signature of epithelial-mesenchymal transition in breast cancer

ABSTRACT: Epithelial-mesenchymal transition (EMT), a switch of polarized epithelial cells to a migratory, fibroblastoid phenotype, is considered a key process driving tumor cell invasiveness and metastasis. Using breast cancer cell lines as a model system, we sought to discover gene-expression signatures of EMT with clinical and mechanistic relevance. A supervised comparison of epithelial and mesenchymal breast cancer lines defined a 200-gene EMT signature that was prognostic across multiple breast cancer cohorts. Immunostaining of LYN, a top-ranked EMT signature gene and Src-family tyrosine kinase, was associated with significantly shorter overall survival (P=0.02), and correlated with the basal-like (“triple-negative”) phenotype. In mesenchymal breast cancer lines, RNAi-mediated knockdown of L

ORGANISM(S): Homo sapiens

SUBMITTER: Jonathan Pollack 

PROVIDER: E-GEOD-13915 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress