MelR experiment 1
Ontology highlight
ABSTRACT:
ORGANISM(S): Escherichia coli
SUBMITTER: Jon Hobman
PROVIDER: E-GEOD-1421 | biostudies-arrayexpress |
REPOSITORIES: biostudies-arrayexpress
Publications
Genomic studies with Escherichia coli MelR protein: applications of chromatin immunoprecipitation and microarrays.
Grainger David C DC Overton Timothy W TW Reppas Nikos N Wade Joseph T JT Tamai Eiji E Hobman Jon L JL Constantinidou Chrystala C Struhl Kevin K Church George G Busby Stephen J W SJ
Journal of bacteriology 20041001 20
Escherichia coli MelR protein is a transcription activator that is essential for melibiose-dependent expression of the melAB genes. We have used chromatin immunoprecipitation to study the binding of MelR and RNA polymerase to the melAB promoter in vivo. Our results show that MelR is associated with promoter DNA, both in the absence and presence of the inducer melibiose. In contrast, RNA polymerase is recruited to the melAB promoter only in the presence of inducer. The MelR DK261 positive control ...[more]
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