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The expression profile of Escherichia coli K-12 in response to minimal, optimal and excess copper concentrations


ABSTRACT: The gene expression profile of E. coli K-12 MG1655 grown in minimal medium supplemented with elevated copper concentrations (as copper-glycine) has been analysed using whole genome oligonucleotide microarrays. â??Poolâ?? RNA was isolated from five independently grown 50ml DMA cultures of E. coli K-12 MG1655. â??Testâ?? RNA was isolated from three independently grown 50ml DMA cultures of E. coli K-12 MG1655, for each growth condition (no added CuSO4, or supplemented with either 0.75mM or 2mM copper glycine). A sample of each culture was taken at mid to late exponential growth phase at OD600 0.8 (measured using a Pharmacia Biotech Ultrospec 2000 in a cuvette with a path length of 10mm).

ORGANISM(S): Escherichia coli

SUBMITTER: Jon Hobman 

PROVIDER: E-GEOD-1780 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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The expression profile of Escherichia coli K-12 in response to minimal, optimal and excess copper concentrations.

Kershaw Christopher J CJ   Brown Nigel L NL   Constantinidou Chrystala C   Patel Mala D MD   Hobman Jon L JL  

Microbiology (Reading, England) 20050401 Pt 4


The gene expression profile of Escherichia coli K-12 MG1655 grown in minimal medium supplemented with elevated copper concentrations (as copper-glycine) has been analysed using whole-genome oligonucleotide microarrays. At 750 muM copper-glycine, the expression of both the cue and cus copper-export systems is evident. At near-lethal copper concentrations (2 mM copper-glycine), the expression of these two regulons increases significantly. Other regulons with increased transcription in response to  ...[more]

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