Gene expression profiling of three paired parental and cisplatin-resistant TGCT cell lines

ABSTRACT: The development of chemo-resistance has dramatically limited the clinical efficiency of platinum-based therapy. Although many resistant mechanisms have been demonstrated, genetic/molecular alterations responsible for drug resistance in the majority of clinical cases has not been identified. We analyzed three pairs of testicular germ cell tumor (TGCT) cell lines using Affymetrix expression microarrays to identify differential expressed genes. Then the expression of CCND1/CyclinD1, selected from the microarray analysis, was determined in cisplatin sensitive and resistance cancer samples including TGCTs, ovarian and prostate cancers by quantitative reverse transcription PCR analysis (qRT-PCR). Finally, we determined the gene knocked-down effect of CyclinD1. Expression microarray study rev

ORGANISM(S): Homo sapiens

SUBMITTER: YONG-JIE LU 

PROVIDER: E-GEOD-14231 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress