Cell cycle-regulated gene expression in tobacco cells

ABSTRACT: Tobacco BY-2 cells can be highly synchronized by treatment with an inhibitor of DNA synthesis, aphidicolin. For comprehensive understanding of cell cycle-regulated gene expression in tobacco, microarray analysis was conducted in synchronized cultures of BY-2 cells. Cells were treated with aphidicolin for 24 hours to block the entry into and progression through the S phase. After release from the aphidicolin block, cells were sampled every 2 h from 0–16 h. cDNA probes prepared from these samples were hybridised to a custom-made16K microarray. We performed this procedure twice independently to identify transcripts that reproducibly showed similar cell cycle-regulated expression. A total of 883 putative cell cycle-regulated transcripts were identified from 16,896 duplicated spots on the mic

ORGANISM(S): Nicotiana tabacum

SUBMITTER: Hiroo Fukuda 

PROVIDER: E-GEOD-14250 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress