Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

Cell cycle-regulated gene expression in tobacco cells

ABSTRACT: Tobacco BY-2 cells can be highly synchronized by treatment with an inhibitor of DNA synthesis, aphidicolin. For comprehensive understanding of cell cycle-regulated gene expression in tobacco, microarray analysis was conducted in synchronized cultures of BY-2 cells. Cells were treated with aphidicolin for 24 hours to block the entry into and progression through the S phase. After release from the aphidicolin block, cells were sampled every 2 h from 0â16 h. cDNA probes prepared from these samples were hybridised to a custom-made16K microarray. We performed this procedure twice independently to identify transcripts that reproducibly showed similar cell cycle-regulated expression. A total of 883 putative cell cycle-regulated transcripts were identified from 16,896 duplicated spots on the microarrays. These transcripts could be categorised into 10 classes on the basis of transcript expression patterns in the cell cycle. Keywords: time course Synchronization experiments were repeated twice (experiments A and B). In each experiment, cells were sampled every 2 h during the culture periods (0-16 h), and used for microarray analysis.

ORGANISM(S): Nicotiana tabacum

SUBMITTER: Hiroo Fukuda

PROVIDER: E-GEOD-14250 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Preferential up-regulation of G2/M phase-specific genes by overexpression of the hyperactive form of NtmybA2 lacking its negative regulation domain in tobacco BY-2 cells.

Kato Kiichi K Gális Ivan I Suzuki Shiori S Araki Satoshi S Demura Taku T Criqui Marie-Claire MC Potuschak Thomas T Genschik Pascal P Fukuda Hiroo H Matsuoka Ken K Ito Masaki M

Plant physiology 20090225 4

Many G2/M phase-specific genes in plants contain mitosis-specific activator (MSA) elements, which act as G2/M phase-specific enhancers and bind with R1R2R3-Myb transcription factors. Here, we examined the genome-wide effects of NtmybA2 overexpression, one of the R1R2R3-Myb transcription factors in tobacco (Nicotiana tabacum). We used a custom-made 16-K cDNA microarray for comparative transcriptome analysis of transgenic tobacco BY-2 cell lines that overexpress NtmybA2 or its truncated hyperactiv ...[more]

PMID: 19244455

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Dataset Information

Cell cycle-regulated gene expression in tobacco cells

Publications

Preferential up-regulation of G2/M phase-specific genes by overexpression of the hyperactive form of NtmybA2 lacking its negative regulation domain in tobacco BY-2 cells.

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