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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Distinct transcriptional outputs associated with mono- and di-methylated histone H3 arginine 2

ABSTRACT: Dimethylation of histone H3 arginine 2 (H3R2me2) maintains transcriptional silencing by inhibiting Set-1 mediated trimethylation of H3K4. Here we demonstrate that arginine 2 is also monomethylated (H3R2me1) in yeast but that its functional characteristics are distinct from H3R2me2: (a) H3R2me1 does not inhibit H3K4 methylation, (b) it is present throughout the coding region of genes and (c) it correlates with active transcription. Collectively, these results indicate that different H3R2 methylated states have defined roles in gene expression. hIP-chip analysis was performed to determine the genomic distribution of histone modifications H3R2me1, H3R2me2a, H3K4me3, H3K36me3, and H3K79me3 in the BY4741 yeast strain. ChIP samples were amplified, labelled, and hybridized to 50-mer tiling arrays covering the entire yeast genome at a 64 bp resolution.

ORGANISM(S): Saccharomyces cerevisiae

SUBMITTER: Helena Santos-Rosa

PROVIDER: E-GEOD-14453 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Distinct transcriptional outputs associated with mono- and dimethylated histone H3 arginine 2.

Kirmizis Antonis A Santos-Rosa Helena H Penkett Christopher J CJ Singer Michael A MA Green Roland D RD Kouzarides Tony T

Nature structural & molecular biology 20090308 4

Dimethylation of histone H3 Arg2 (H3R2me2) maintains transcriptional silencing by inhibiting Set1 mediated trimethylation of H3K4. Here we demonstrate that Arg2 is also monomethylated (H3R2me1) in yeast but that its functional characteristics are distinct from H3R2me2: (i) H3R2me1 does not inhibit histone H3 Lys4 (H3K4) methylation; (ii) it is present throughout the coding region of genes; and (iii) it correlates with active transcription. Collectively, these results indicate that different H3R2 ...[more]

PMID: 19270702

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