Distinctive responses to nitrogen starvation in the dominant active mutants of the fission yeast Rheb GTPase.

ABSTRACT: Rheb, a ras-like small GTPase conserved from human to yeast, controls Tor kinase and plays a central role in regulation of cell growth depending on extracellular conditions. Fission yeast Rheb regulates amino acid uptake as well as response to nitrogen starvation. In this study we generated two mutants of Rheb, rhb1-DA4 and rhb1-DA8, and characterized them genetically. V17A mutation within the G1 box defined for the ras-like GTPases was responsible for rhb1-DA4, and Q52R I76F within the switch II domain for rhb1-DA8. In fission yeast, two events, induction of a meiosis initiating gene mei2+ and cell division without cell growth, are a typical response to nitrogen starvation. Under nitrogen-rich conditions, Rheb stimulates Tor kinase, which, in turn, suppresses the response to nitrogen star

ORGANISM(S): Schizosaccharomyces pombe

SUBMITTER: Yuji Chikashige 

PROVIDER: E-GEOD-14626 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress