Accumulation of sumoylated Rad52 in checkpoint mutants perturbed in DNA replication

ABSTRACT: Checkpoints are cellular surveillance and signaling pathways that regulate responses to DNA damage and perturbations of DNA replication. Here we show that high levels of sumoylated Rad52 are present in the mec1 sml1 and rad53 sml1 checkpoint mutants exposed to DNA damaging agents such as methyl methanesulfonate (MMS) or the DNA replication inhibitor hydroxyurea (HU). The kinase-defective mutant rad53-K227A also showed high levels of Rad52 sumoylation. Elevated levels of Rad52 sumoylation occur in checkpoint mutants proceeding S phase being exposed DNA-damaging agent. Interestingly, ChIP on chip analyses revealed non-canonical chromosomal localization of Rad52 in the HU-treated rad53-K227A cells arrested in early S phase: Rad52 localization at dormant and early DNA replication origins. Howe

ORGANISM(S): Saccharomyces cerevisiae

SUBMITTER: Takashi Ohuchi 

PROVIDER: E-GEOD-14761 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress