Methylation profiling of human embryonic stems cells and developing human fetal liver reveals distinct dna methylation patterns characterize these samples

ABSTRACT: Publication title: Distinct DNA methylation patterns characterize differentiated human embryonic stem cells and developing human fetal liver; To investigate the role of DNA methylation during human development, we developed Methyl-seq, a method that assays DNA methylation at more than 90,000 regions throughout the genome. Performing Methyl-seq on human embryonic stem cells (hESCs), their derivatives and human tissues allowed us to identify several trends during hESC and in vivo liver differentiation. First, differentiation results in DNA methylation changes at a minimal number of assayed regions, both in vitro and in vivo (2-11%). Second, in vitro hESC differentiation is characterized by both de novo methylation and demethylation, whereas in vivo fetal liver development is characterize

ORGANISM(S): Homo sapiens

SUBMITTER: Rami Rauch 

PROVIDER: E-GEOD-14966 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress