Subtractive Hybridization Reveals Novel Genes Associated with D. melanogaster Early Embryogenesis.

ABSTRACT: Background: The early stages of D. melanogaster embryogenesis involve cell migration and pattern formation, and lead to the formation of three germ layers (the ectoderm, mesoderm and endoderm). These developmental events are controlled by differential gene activity. In the current study we used a suppressive subtractive hybridization (SSH) procedure to identify a group of genes potentially involved in D. melanogaster early embryogenesis and to study the temporal activity of developmentally regulated genes at five different intervals covering 12 stages of embryogenesis. Results: Macroarrays were constructed to confirm induction of expression and determine the temporal profile of isolated subtracted cDNAs during embryo development. We identified a set of 118 genes that significantly increase

ORGANISM(S): Drosophila melanogaster

SUBMITTER: Pablo Moreno 

PROVIDER: E-GEOD-15000 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress