Transcription profiling of Arabidopsis laser microdissected cells at the powdery mildew infection site

ABSTRACT: Obligate biotrophs such as the virulent powdery mildew Golovinomyces orontii alter plant host cellular architecture, metabolism, and defense in order to acquire nutrients while suppressing cell death and senescence. G. orontii exclusively infects epidermal cells of Arabidopsis with clearly defined stages of infection. Host factors mediating the powdery mildew (PM) interaction are often expressed in the mesophyll cells underlying the infected epidermal cells. Therefore, in order to identify Arabidopsis processes and regulators mediating this PM interaction, we used UV laser microdissection to isolate cells at the PM infection site for global expression profiling. As part of this process, we optimized and/or developed novel tissue preparation, RNA extraction and amplification, and quality co

ORGANISM(S): Arabidopsis thaliana

SUBMITTER: Mary Wildermuth 

PROVIDER: E-GEOD-15680 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress