Halobacterium sp. NRC-1 ChIP-chip for TFBa, TFBd and TFBf, high resolution array

ABSTRACT: A detailed map of genomic locations where TFs bind DNA and modulate transcription is essential to model mechanisms of gene regulation on a systems-scale. Chromatin immunoprecipitation of transcription complexes coupled to microarray (ChIP-chip (Ren et al, 2000)) or sequencing (ChIP-seq (Robertson et al, 2007)) is a commonly used approach to construct such maps. In ChIP-chip, the resolution to which the protein-DNA binding sites (TFBSs) can be identified is often limited by the genomic spacing of the probes in the array. We utilized the MeDiChI algorithm (Reiss et al, 2008) to estimate precise TFBS locations and their corresponding local false discovery rates (LFDRs) from high-resolution arrays for TFBa, TFBd and TFBf. This regression-based method deconvolves the ChIP-chip enrichment ratios

ORGANISM(S): Halobacterium sp. NRC-1

SUBMITTER: David Reiss 

PROVIDER: E-GEOD-15786 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress