Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

0

An mRNA/3' UTR-directed primary piRNA pathway in Drosophila ovarian somatic cells

ABSTRACT: Piwi-interacting RNAs (piRNAs) are ~24-30 nucleotide regulatory RNAs that are abundantly expressed in gonads. The most well-understood piRNAs mediate post-transcriptional defense against transposable elements (TEs), and derive from sense or antisense strands as a consequence of "ping-pong" amplification of complementary sequences of active TEs and piRNA master control transcripts. Another class of piRNAs, such as those expressed in pachytene testis, derive from large intergenic clusters that are strictly single-stranded. Here, we report a third substrate that generates abundant primary piRNAs. In somatic follicle cells of Drosophila ovaries, we cloned >1 million piRNAs from thousands of messenger RNAs, and these were quite preferentially derived from 3' untranslated regions. This segregation implies a competition between the translation machinery and primary piRNA biogenesis machinery for mRNA access. 3 replicates.

ORGANISM(S): Drosophila melanogaster

SUBMITTER: Nicolas Robine 

PROVIDER: E-GEOD-15825 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

Json Xml
altmetric image

Publications

A broadly conserved pathway generates 3'UTR-directed primary piRNAs.

Robine Nicolas N   Lau Nelson C NC   Balla Sudha S   Jin Zhigang Z   Okamura Katsutomo K   Kuramochi-Miyagawa Satomi S   Blower Michael D MD   Lai Eric C EC  

Current biology : CB 20091201 24

<h4>Background</h4>Piwi-interacting RNAs (piRNAs) are approximately 24-30 nucleotide regulatory RNAs that are abundant in animal gonads and early embryos. The best-characterized piRNAs mediate a conserved pathway that restricts transposable elements, and these frequently engage a "ping-pong" amplification loop. Certain stages of mammalian testis also accumulate abundant piRNAs of unknown function, which derive from noncoding RNAs that are depleted in transposable element content and do not engag  ...[more]

Similar Datasets

Unknown An mRNA/3' UTR-directed primary piRNA pathway in Drosophila ovarian somatic cells
2010-01-04 | GSE15825 | GEO
Transcriptomics Euchromatic transposon insertions trigger production of novel pi- and endo-siRNAs at the target sites in the Drosophila germline
2014-02-07 | E-GEOD-46105 | biostudies-arrayexpress
Transcriptomics Shutdown is a component of the Drosophila piRNA biogenesis machinery (RNA-seq)
2012-06-30 | E-GEOD-38090 | biostudies-arrayexpress
Transcriptomics piRNA-mediated transgenerational inheritance of an acquired trait
2012-04-13 | E-GEOD-34506 | biostudies-arrayexpress
Transcriptomics A role for Fkbp6 and the chaperone machinery in piRNA amplification and transposon silencing
2012-09-04 | E-GEOD-39203 | biostudies-arrayexpress
Unknown Euchromatic transposon insertions trigger production of novel pi- and endo-siRNAs at the target sites in the Drosophila germline
2014-02-07 | GSE46105 | GEO
Proteomics Coupled protein synthesis and ribosome-guided piRNA processing on mRNAs
2021-09-17 | PXD027489 | Pride
Unknown RNF17 blocks promiscuous activity of PIWI proteins in mouse testes
2014-12-17 | GSE53919 | GEO
Unknown RNF17 blocks promiscuous activity of PIWI proteins in mouse testes [5'RACE]
2014-12-17 | GSE53915 | GEO
Transcriptomics Krimper enforces an antisense bias on piRNA pools by binding AGO3 in the Drosophila germline
2015-05-28 | E-GEOD-69293 | biostudies-arrayexpress