Browse
Submit Data
Databases
API
Help

Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

12 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Expression analysis for nasopharyngeal carcinoma cell lines (5-8F and 6-10B)

ABSTRACT: High metastatic nasopharyngeal carcinoma cell line 5-8F expression patterns against low metastatic nasopharyngeal carcinoma cell line 6-10B. This study was done to screen genes related with metastasis in NPC. The microarray analyses were done in a same batch. 6-10B cells are cultured and harvested at three different time points.

ORGANISM(S): Homo sapiens

SUBMITTER: Zhong-Xi Huang

PROVIDER: E-GEOD-15903 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Json Xml

Publications

Microarray analysis of differentially expressed genes between nasopharyngeal carcinoma cell lines 5-8F and 6-10B.

Li Jia J Fan Ying Y Chen Jing J Yao Kai-Tai KT Huang Zhong-Xi ZX

Cancer genetics and cytogenetics 20100101 1

Nasopharyngeal carcinoma (NPC) cell lines 5-8F (high tumorigenic and metastatic) and 6-10B (low tumorigenic and metastatic) are subclones of SUNE1. To address their biological differences, three biologic repeats of expression microarray analysis were performed. Only 60 differently expressed genes were identified between the two cell lines. These genes were randomly distributed on all the chromosomes. Gene ontology analysis showed that most of these genes participated in cellular and metabolic pr ...[more]

PMID: 19963132

Similar Datasets

Expression analysis for nasopharyngeal carcinoma cell lines 5-8F

Project description:High metastatic nasopharyngeal carcinoma cell line 5-8F expression patterns. This study was done to detect genes expressed in NPC cell line 5-8F. The microarray analyses were done in a same batch. cells are cultured and harvested at two different time points.

2009-12-10 | E-GEOD-15921 | biostudies-arrayexpress

Expression analysis for nasopharyngeal carcinoma cell lines (5-8F and 6-10B)

Project description:High metastatic nasopharyngeal carcinoma cell line 5-8F expression patterns against low metastatic nasopharyngeal carcinoma cell line 6-10B.

2009-12-10 | GSE15903 | GEO

Nasopharyngeal carcinoma cell lines (copy number analysis)

Project description:Five NPC cell lines (HONE1, CNE1, CNE2, 5-8F, and 6-10B) and an immortalized nasopharyngeal epithelial cell line NP69 were evaluated with 250K SNP arrays (Affymetrix) to detect the genome-wide DNA copy number alterations. The same type of data of 45 Chinese samples from the HapMap project was used as normal control. Data was analyzed using GCOS (GeneChip operating software, Ver.1.4), GTYPE (GeneChip Genotyping Analysis Software, Ver.4.1), and CNAT (chromosome copy number analysis tool, Ver.4.0, BRLMM algorithm).

2009-03-04 | E-GEOD-15051 | biostudies-arrayexpress

Nasopharyngeal carcinoma cell lines (expression analysis)

Project description:Three nasopharyngeal carcinoma cell lines (CNE1, CNE2, and HONE1) expression patterns against an immortalized nasopharyngeal epithelial cell line NP69. This study was done to screen genes consensually overexpressed in NPC. NP69 was used as normal control. The microarray analyses were done in a same batch.

2009-03-05 | E-GEOD-15047 | biostudies-arrayexpress

Expression analysis for nasopharyngeal carcinoma cell lines 5-8F

Project description:High metastatic nasopharyngeal carcinoma cell line 5-8F expression patterns.

2009-12-10 | GSE15921 | GEO

Nasopharyngeal carcinoma cell lines (expression analysis and copy number analysis)

Project description:This SuperSeries is composed of the following subset Series: GSE15047: Nasopharyngeal carcinoma cell lines (expression analysis) GSE15051: Nasopharyngeal carcinoma cell lines (copy number analysis) This study was designed to screen genes overexpression due to copy number gain.

2009-03-04 | E-GEOD-15098 | biostudies-arrayexpress

Nasopharyngeal carcinoma cell lines (copy number analysis)

2009-03-05 | GSE15051 | GEO

Expression data for nasopharyngeal carcinoma cell line 5-8F stably expressing either LTF gene or empty vector.

Project description:LTF (lactotransferrin, lactoferrin) is a key component of innate immune defense. It has recently been found to have antitumor and antimetastatic activity in different cancers. We previously reported LTF to be the most significantly downregulated gene in nasopharyngeal carcinoma (NPC) specimens relative to normal nasopharyngeal epithelial tissues and it was also negatively associated with the progression and metastasis of NPC. However, the mechanism underlying this remains unclear. In the current study, we performed genome expression profiling analysis using the Affymetrix genome wide microarray system to identify broad scale changes in gene expression associated with LTF gene overexpression in NPC cell line 5-8F. NPC cell line5-8F were transfected with either PIRES empty vector (Clontech) or PIRES-LTF vector by Lipofectamine 2000 (Invitrogen). Cells stably expressing either LTF or empty vector were generated by selection with growth medium supplemented with 400 M-NM-<g/mL G418. When cells were growing at 80% confluence, total RNA were extracted and detected by Affymerix GeneChip Human Gene U133 plus2.0.

2013-12-26 | E-GEOD-36972 | biostudies-arrayexpress

Expression data for nasopharyngeal carcinoma cell line 5-8F stably expressing either LTF gene or empty vector.

2013-12-26 | GSE36972 | GEO

MicroRNA-18a promotes cancer progression through SMG1 suppression and mTOR pathway activation in nasopharyngeal carcinoma

Project description:To search for molecular processes underlying miR-18a-mediated oncogenesis in nasopharyngeal carcinoma(NPC) cells, whole-genome mRNA microarray analysis was performed using the HumanGene Expression 4 x 44k v2 Microarray Kit (Agilent Technologies) in 6-10B cells with stable miR-18a overexpression and in 5-8F cells with transient silencing of miR-18a by siRNA. Differentially expressed genes in miR-18a-overexpressing cell and differentially expressed genes in miR-18a knockdown cells were analyzed.

2019-12-25 | GSE137689 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data