TAcKLE - Transcriptome Amplification and cDNA Labeling for Expression analysis

ABSTRACT: To characterize the properties and evaluate the performance of the TAcKLE procedure, a novel method providing effective transcriptome amplification for expression analyses on oligonucleotide microarrays, we performed 20 two-color hybridizations using self-spotted Operon 27k arrays. A single source of universal human reference RNA (pooled from 10 cell lines) and RNA extracted from healthy breast tissue was used for all experiments to avoid differences in transcript abundance imposed by the RNA preparation. RNA was either amplified by one or two rounds of linear isothermal RNA amplification (starting from 2000 ng, 200 ng, 20 ng or 2 ng), followed by Cy-dUTP incorporation using Klenow fragment, or labeled directly by reverse transcription of 40 µg RNA with Cy-dUTP. For all quantities of start

ORGANISM(S): Homo sapiens

SUBMITTER: Joerg Schlingemann 

PROVIDER: E-GEOD-1645 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress