Expression profiling of heat stress response in peanut using oligonucleotide microarrays

ABSTRACT: To gain insights into molecular mechanisms of tolerance to heat stress, we conducted a transcript profiling experiment to identify heat-responsive genes in contrasting peanut mini core accessions, either un-acclimated or acclimated to heat stress. Plants at reproductive stage were exposed to 28 °C (control), 45 °C for 15 d (un-acclimated) or 45 °C for 1 d followed by 7 d recovery and 15 d stress (acclimated). Two contrasting genotypes showing diverse response to stress were selected based on a bioassay involving chlorophyll fluorescence yield under elevated respiratory demand and membrane thermostability. Transcript profiling was performed using 4 x 44k custom oligo microarrays containing 22k peanut EST sequences. The microarray analysis identified 710 stress-induced and 770 stress-rep

ORGANISM(S): Arachis hypogaea

SUBMITTER: Kameswara Rao Kottapalli 

PROVIDER: E-GEOD-16492 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress