Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Expression data from androgen treated LNCaP cells

ABSTRACT: Androgens are required for the development of normal prostate, and they are also linked to the development of prostate cancer. We used microarrays to understand the role of androgen in an androgen dependent, androgen receptor (AR) positive human metastatic cell line, LNCaP. LNCaP cells were grown in RPMI medium and they were subjected to stay in phenol-red free, RPMI with charcoal stripped serum for 48h. Synthetic androgen R1881 was added and the cells were allowed to grow for 48h. Control cells were given with corresponding amount of ethanol as vehicle which is used for the solubilization of R1881. Cells were harvested and RNA was isolated for microarray analysis.

ORGANISM(S): Homo sapiens

SUBMITTER: Adaikkalam Vellaichamy 

PROVIDER: E-GEOD-17044 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Proteomic interrogation of androgen action in prostate cancer cells reveals roles of aminoacyl tRNA synthetases.

Vellaichamy Adaikkalam A   Sreekumar Arun A   Strahler John R JR   Rajendiran Theckelnaycke T   Yu Jindan J   Varambally Sooryanarayana S   Li Yong Y   Omenn Gilbert S GS   Chinnaiyan Arul M AM   Nesvizhskii Alexey I AI  

PloS one 20090918 9

Prostate cancer remains the most common malignancy among men in United States, and there is no remedy currently available for the advanced stage hormone-refractory cancer. This is partly due to the incomplete understanding of androgen-regulated proteins and their encoded functions. Whole-cell proteomes of androgen-starved and androgen-treated LNCaP cells were analyzed by semi-quantitative MudPIT ESI- ion trap MS/MS and quantitative iTRAQ MALDI- TOF MS/MS platforms, with identification of more th  ...[more]

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