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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Multiple Sclerosis Blood Cell mRNA Transcriptome

ABSTRACT: We measured mRNA expression for all known genes in whole blood from 144 individuals, 99 with MS (43 PPMS, 36 RRMS and 20 SPMS). Analysis of gene expression and genotype in these individuals has indicated the dominant genetic association with T cell regulation is matched by the dominant pattern of dysregulation of T cell gene expression in MS and its clinical subtypes. Whole blood mRNA expression was compared between different types of MS and age-matched healthy control to identify differentially expressed genes between them. The supplementary file 'GSE17048_non-normalized_data.txt' contains non-normalized data for Samples GSM426595-GSM426738.

ORGANISM(S): Homo sapiens

SUBMITTER: Kaushal Gandhi

PROVIDER: E-GEOD-17048 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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The multiple sclerosis whole blood mRNA transcriptome and genetic associations indicate dysregulation of specific T cell pathways in pathogenesis.

Gandhi Kaushal S KS McKay Fiona C FC Cox Mathew M Riveros Carlos C Armstrong Nicola N Heard Robert N RN Vucic Steve S Williams David W DW Stankovich Jim J Brown Matthew M Danoy Patrick P Stewart Graeme J GJ Broadley Simon S Moscato Pablo P Lechner-Scott Jeannette J Scott Rodney J RJ Booth David R DR

Human molecular genetics 20100227 11

Multiple sclerosis (MS) is an autoimmune disease with a genetic component, caused at least in part by aberrant lymphocyte activity. The whole blood mRNA transcriptome was measured for 99 untreated MS patients: 43 primary progressive MS, 20 secondary progressive MS, 36 relapsing remitting MS and 45 age-matched healthy controls. The ANZgene Multiple Sclerosis Genetics Consortium genotyped more than 300 000 SNPs for 115 of these samples. Transcription from genes on translational regulation, oxidati ...[more]

PMID: 20190274

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Multiple Sclerosis Blood Cell mRNA Transcriptome

Project description:We measured mRNA expression for all known genes in whole blood from 144 individuals, 99 with MS (43 PPMS, 36 RRMS and 20 SPMS). Analysis of gene expression and genotype in these individuals has indicated the dominant genetic association with T cell regulation is matched by the dominant pattern of dysregulation of T cell gene expression in MS and its clinical subtypes.

2010-04-02 | GSE17048 | GEO

Novel Biomarkers and Interferon Signature in Secondary Progressive Multiple Sclerosis

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CSF B cells in relapsing multiple sclerosis are driven towards an antigen-experienced, inflammatory fate

Project description:Central nervous system B cells have several potential roles in multiple sclerosis (MS): secretors of proinflammatory cytokines and chemokines, presenters of autoantigens to T cells, producers of pathogenic antibodies, and reservoirs for viruses that trigger demyelination. To interrogate these roles, single-cell RNA sequencing (scRNA-Seq) was performed on paired cerebrospinal fluid (CSF) and blood from subjects with relapsing-remitting MS (RRMS; n = 12), other neurologic diseases (ONDs; n = 1), and healthy controls (HCs; n = 3). Single-cell immunoglobulin sequencing (scIg-Seq) was performed on a subset of these subjects and additional RRMS (n = 4), clinically isolated syndrome (n = 2), and OND (n = 2) subjects. Further, paired CSF and blood B cell subsets (RRMS; n = 7) were isolated using fluorescence activated cell sorting for bulk RNA sequencing (RNA-Seq). Independent analyses across technologies demonstrated that nuclear factor kappa B (NF-?B) and cholesterol biosynthesis pathways were activated, and specific cytokine and chemokine receptors were up-regulated in CSF memory B cells. Further, SMAD/TGF-ß1 signaling was down-regulated in CSF plasmablasts/plasma cells. Clonally expanded, somatically hypermutated IgM+ and IgG1+ CSF B cells were associated with inflammation, blood–brain barrier breakdown, and intrathecal Ig synthesis. While we identified memory B cells and plasmablast/plasma cells with highly similar Ig heavy-chain sequences across MS subjects, similarities were also identified with ONDs and HCs. No viral transcripts, including from Epstein–Barr virus, were detected. Our findings support the hypothesis that in MS, CSF B cells are driven to an inflammatory and clonally expanded memory and plasmablast/plasma cell phenotype.

2020-08-31 | GSE133028 | GEO

Expression data of multiple sclerosis patients receiving Interferon-beta therapy [miRNA-2_0]

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Expression data of multiple sclerosis patients receiving Interferon-beta therapy [HG-U133_Plus_2]

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Expression data of multiple sclerosis patients receiving Interferon-beta therapy [miRNA-2_0]

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Expression data of multiple sclerosis patients receiving Interferon-beta therapy [HG-U133_Plus_2]

2013-04-24 | GSE46280 | GEO

scRNAseq of blood and CSF CD4+ T cells from MS patients and controls

Project description:To study the regulation of candidate genes from our study in human cells, we analyzed CD4+ T cells from blood and CSF of MA patients and age and sex matched idiopathic intracranial hypertension controls We analyzed 40845 cells in control blood, 807 cells in control CSF, 29749 cells in MS blood and 15768 cells in MS CSF

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Expression data of multiple sclerosis patients receiving Interferon-beta therapy [TaqMan(r) Array Human MicroRNA B Cards v2.0]

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Expression data of multiple sclerosis patients receiving Interferon-beta therapy [TaqMan(r) Array Human MicroRNA A Cards v2.0]

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