Expression data from RNA half-life experiments in Prochlorococcus

ABSTRACT: RNA decay was measured in Prochlorococcus after inhibition of transcription by rifampicin using customized Affymetrix gene expression arrays. RNA turnover plays an important role in the gene regulation of microorganisms and influences their speed of acclimation to environmental changes. We investigated whole-genome RNA stability of Prochlorococcus, a relatively slow-growing marine cyanobacterium doubling approximately once a day, which is extremely abundant in the oceans. Using a combination of microarrays, quantitative RT-PCR and a new algorithm for determining RNA decay rates, we found a median half-life of 2.4 min and a median decay rate of 2.6 min for expressed genes – two-fold faster than that reported for any organism. The shortest transcript half-life (33 seconds) was for a gene o

ORGANISM(S): Prochlorococcus marinus subsp. pastoris str. CCMP1986

SUBMITTER: Trent Rector 

PROVIDER: E-GEOD-17075 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress