Reprogramming of a B cell line into macrophages

ABSTRACT: Transcription factor induced reprogramming of one specialized cell type into another is a promising approach for regenerative medicine. However, the process still remains poorly understood, in large part because of the lack of adequate experimental models. Here we describe a robust cell reprogramming system consisting of a B cell line with an inducible form of C/EBPa that can be converted into macrophages with essentially 100% efficiency in only 2 to 3 days. The conversion involves reciprocal changes in cell surface antigen expression, increase in cell granularity and size, alterations in cellular structures, formation of membrane extensions, acquisition of phagocytic capacity and an increased inflammatory responsiveness as well as migratory activity. Analysis of the transcriptome shows co

ORGANISM(S): Mus musculus

SUBMITTER: Thomas GRAF 

PROVIDER: E-GEOD-17316 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress