Let-7 microRNAs are developmentally regulated in circulating human erythroid cells

ABSTRACT: MicroRNA are ~22nt-long small non-coding RNA that negatively regulate protein expression through mRNA degradation or translational repression in eukaryotic cells. Based upon their importance in regulating development and terminal differentiation in model systems, erythrocyte microRNA profiles were examined at birth and in adults to determine if changes in their abundance coincide with the developmental phenomenon of hemoglobin switching. Expression profiling of microRNA was performed using total RNA from 4 adult peripheral blood samples compared to 4 cord blood samples after depletion of plasma, platelets, and nucleated cells. Labeled RNA was hybridized to custom spotted array containing 474 human microRNA species (miRBase release 9.1). Total RNA from Epstein-Barr virus (EBV)-transformed l

ORGANISM(S): Human herpesvirus 4

SUBMITTER: Francesco Marincola 

PROVIDER: E-GEOD-17405 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress