Identification of transcription factor EOR-1 binding sites in C.elegans

ABSTRACT: We, being members of modENCODE consortium, have established an experimetal pipeline in C.elegans that allows global identification of the binding sites for transcription factors using chromatin immunoprecipitation followed by illumina high-throughput sequencing (ChIP-seq). In current study, we identified the binding sites for EOR-1 at L3 stage, which plays important roles in regulating RAS/RAF-mediated signaling during excretory system development and RAS/RAF- and WNT-mediated signaling during P12 fate specification. For data usage terms and conditions, please refer to http://www.genome.gov/27528022 and http://www.genome.gov/Pages/Research/ENCODE/ENCODEDataReleasePolicyFinal2008.pdf EOR-1 was tagged with a dual GFP:3xFLAG tag at the carboxy terminus, whose expression pattern was confirme

ORGANISM(S): Caenorhabditis elegans

SUBMITTER: Michael Wilson 

PROVIDER: E-GEOD-17456 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress