RNA-seq analysis of the transcriptome from Sulfur Deprivation Chlamydomonas cells

ABSTRACT: The Chlamydomonas reinhardtii transcriptome was characterized from nutrient-replete and sulfur-depleted wild-type and snrk2.1 mutant cells; the mutant is null for the regulatory serine-threonine kinase SNRK2.1, which is required for acclimation to sulfur deprivation. The transcriptome analyses involved microarray hybridization and RNA-seq technology; RT-qPCR evaluation of the data obtained by these techniques showed that RNA-seq is significantly more quantitative than microarray hybridizations. Sulfur-deprivation-responsive transcripts included those encoding proteins involved in sulfur acquisition and assimilation, recycling of sulfur-containing amino acids, synthesis of reduced sulfur metabolites and cofactors, and modification of cellular structures such as the cell wall and complexes a

ORGANISM(S): Chlamydomonas reinhardtii

SUBMITTER: Arthur Grossman 

PROVIDER: E-GEOD-17970 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress