Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Three acyltransferases and a nitrogen responsive regulator are implicated in nitrogen starvation-induced triacylglycerol accumulation in Chlamydomonas


ABSTRACT: Three acyltransferases, DGAT1, DGTT1, and PDAT1, are induced by nitrogen starvation and are likely to have a role in TAG accumulation based on their patterns of expression. Each gene also shows increased mRNA abundance in other TAG-accumulating conditions (-S, -P, -Zn, -Fe). Insertional mutants, pdat1-1 and pdat1-2, accumulate 25% less TAG compared to the parent strain, D66+, which demonstrates the relevance of the trans-acylation pathway in Chlamydomonas. The biochemical functions of DGTT1 and PDAT1 were validated by rescue of oleic acid sensitivity and restoration of TAG accumulation in a yeast strain lacking all acyltransferase activity. Time course analysis suggest than an SBP domain transcription factor protein, whose mRNA increases precede that of other genes like DGAT1, is a candidate regulator of the N-deficiency response. An insertional mutant, nrr1-1, accumulates only 50% of the TAG compared to the parental strain in N-starvation conditions and is unaffected by other nutrient stresses, suggesting the operation of multiple signaling pathways leading to stress-induced TAG accumulation. Sampling of Chlamydomonas 2137 cultivated in TAP, N-Free TAP or TAP with varying amounts of N.

ORGANISM(S): Chlamydomonas reinhardtii

SUBMITTER: Sabeeha Merchant 

PROVIDER: E-GEOD-34585 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Three acyltransferases and nitrogen-responsive regulator are implicated in nitrogen starvation-induced triacylglycerol accumulation in Chlamydomonas.

Boyle Nanette R NR   Page Mark Dudley MD   Liu Bensheng B   Blaby Ian K IK   Casero David D   Kropat Janette J   Cokus Shawn J SJ   Hong-Hermesdorf Anne A   Shaw Johnathan J   Karpowicz Steven J SJ   Gallaher Sean D SD   Johnson Shannon S   Benning Christoph C   Pellegrini Matteo M   Grossman Arthur A   Merchant Sabeeha S SS  

The Journal of biological chemistry 20120308 19


Algae have recently gained attention as a potential source for biodiesel; however, much is still unknown about the biological triggers that cause the production of triacylglycerols. We used RNA-Seq as a tool for discovering genes responsible for triacylglycerol (TAG) production in Chlamydomonas and for the regulatory components that activate the pathway. Three genes encoding acyltransferases, DGAT1, DGTT1, and PDAT1, are induced by nitrogen starvation and are likely to have a role in TAG accumul  ...[more]

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