Systems-level analysis of N-starvation induced modifications of carbon metabolism in a Chlamydomonas starchless mutant

ABSTRACT: To understand the molecular basis underlying increased triacylglycerol accumulation in starchless Chlamydomonas mutants, we undertook comparative time course transcriptomics of sta6 (CC-4348), a cw strain purported to represent the parental STA6 strain (CC-4349), and 3 independent STA6 strains (STA6-C2, STA6-C4 and STA6-C6, generated by complementation of sta6, CC-4565, M-bM-^@M-^S4566 and M-bM-^@M-^S4567) in the context of nitrogen deprivation. Despite the N starvation induced dramatic remodeling of the transcriptome, there are relatively few differences (5 x 102) between sta6 and STA6, the most dramatic of which are in the abundance of transcripts encoding key regulated or rate-limiting steps in central carbon metabolism, specifically ICL1, MAS1, TAL1, FBP1 and PCK1, suggestive of increa

ORGANISM(S): Chlamydomonas reinhardtii

SUBMITTER: Sabeeha Merchant 

PROVIDER: E-GEOD-51602 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress