Transcription profiling of human HEK293 cells transfected with EWS-FLI1 (Ewing sarcoma family fusion gene)

ABSTRACT: Response of HEK293-cells after transfection with EWS-FLI1. HEK293 cells were transfected with the expression vector pIRES2-EGFP containing type I EWS-FLI1 or empty control vector. For transient transfection standard DEAE dextran method was used and RNA was isolated 48h post transfection. For stable transfection cells were transfected using FuGENE 6 (Roche, Mannheim, Germany) and cells were selected with 400 ug/mL G418. DNA-microarray analysis was performed using Affymetrix HG-U133A microarrays.(see Staege et al. Cancer Res. 2004)

ORGANISM(S): Homo sapiens

DISEASE(S): normal

SUBMITTER: Christoph Hutter 

PROVIDER: E-GEOD-1822 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress